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In view of the complexity of biological processes, real-time analysis and quantification methods have become a necessity to elucidate very complex mechanisms. The aim of the Biosensor workgroup of the GDR IMABIO is to promote reflection and information exchange in order to accelerate the development of these new dynamic imaging approaches in all fields of biology. The meetings of the Biosensor workgroup promote this type of exchange of expertise and federate the biosensing community at the national and European level. This meeting gathers biologists, chemists and physicists developing fluorescent probes, reporters or biosensors either genetically-encoded or synthetic. It will be a unique opportunity for an exchange of ideas and tools over two days.